FastQCFastQC Report
Thu 2 Jun 2016
DRR015190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13785634
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296970.21541990741956446No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT284260.20620016460614002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258790.18772440933801088No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT175700.12745151945859RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149920.1087508924145237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273350.056.1835561
GTATCAA517350.040.792211
GTGGTAT102150.034.053911
ATCAACG656400.031.9773673
TCAACGC665450.031.5392744
TGGTATC107000.031.3833432
TATCAAC677850.031.0996382
CAACGCA724200.029.0261235
AACGCAG749350.028.046
GTACATG712850.025.7471
ACGCAGA831700.025.2306467
TACATGG703250.025.162562
CGCAGAG840750.024.95418
ATGCGGG23200.023.3334248
ACATGGG721750.023.2643493
GCAGAGT895100.023.1793739
CATGGGT230800.022.6844944
AATGCGG25100.021.3855257
CATGGGG312550.020.1567174
GAGTACT477200.019.85147312-13
Produced by FastQC (version 0.11.5)