FastQCFastQC Report
Thu 2 Jun 2016
DRR015189_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015189_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18606334
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315980.16982388900467982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269550.14487002114441244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA340050.054.3745571
GTATCAA632150.039.407571
GTGGTAT136800.032.05921
ATCAACG782000.031.4882163
TCAACGC791400.031.147064
TATCAAC801550.030.8741972
TGGTATC138950.030.2844772
CAACGCA866650.028.4494385
AACGCAG906650.027.279326
GTACATG970250.026.7220651
TACATGG964400.026.255742
ACGCAGA999750.024.6910067
CATGGGT340500.024.4608574
CGCAGAG1008450.024.4496738
ACATGGG996550.024.3662953
GCAGAGT1081000.022.4463029
CATGGGG461900.020.8219344
ATGGGTA155350.019.6373355
CAGAGTA1039550.019.56300210-11
GAGTACA737150.019.2757471
Produced by FastQC (version 0.11.5)