FastQCFastQC Report
Thu 2 Jun 2016
DRR015189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18606334
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341330.18344828164430457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302560.16261129140216446No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT213220.11459538456097801No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG187490.1007667603946054RNA PCR Primer, Index 23 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA348700.054.573211
GTATCAA653450.040.3483051
ATCAACG818250.031.761383
TCAACGC827050.031.4531354
TATCAAC837150.031.3546832
GTGGTAT137100.031.2211931
TGGTATC138250.030.1134932
CAACGCA887750.029.3398865
AACGCAG932550.027.9992056
GTACATG987850.025.943121
TACATGG974200.025.6500112
ACGCAGA1056200.024.6824257
CGCAGAG1059100.024.5856348
CATGGGT346000.024.1232364
ACATGGG1012850.023.5705553
GCAGAGT1136800.022.6779
CATGGGG462900.019.986624
GAGTACA756650.019.5530171
CAGAGTA1101450.019.35844810-11
AGTACAT729700.018.9554652
Produced by FastQC (version 0.11.5)