FastQCFastQC Report
Thu 2 Jun 2016
DRR015188_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015188_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11078714
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283700.2560766529400434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271190.24478472862464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140620.12692808930711633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA288150.056.983771
GTGGTAT104050.040.7979471
TGGTATC108550.038.770442
GTATCAA497400.037.6082731
ATCAACG624100.029.8114263
TATCAAC634900.029.3698082
TCAACGC636050.029.2916494
CAACGCA675050.027.5826345
AACGCAG712100.026.2203486
GTACATG589700.025.6122591
TACATGG580500.025.3822842
ACGCAGA795050.023.447927
ACATGGG604850.023.3657073
CGCAGAG795150.023.357348
GCAGAGT835400.021.8938059
CATGGGT159100.021.2106324
GAGTACT471000.020.63808312-13
CATGGGG302050.020.610824
CAGAGTA804500.020.47780810-11
GTACTTT473750.019.8268914-15
Produced by FastQC (version 0.11.5)