FastQCFastQC Report
Thu 2 Jun 2016
DRR015188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11078714
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317630.2867029512631159No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297150.26821705118482164No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT219700.19830821519537375No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG167770.1514345437566129RNA PCR Primer, Index 39 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157640.14229088321983943No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT135900.12266766702344693RNA PCR Primer, Index 39 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA297550.058.8732531
GTGGTAT100800.042.057651
TGGTATC103750.039.9973342
GTATCAA519950.037.833631
ATCAACG661400.029.6529253
TATCAAC667900.029.5530592
TCAACGC671300.029.2652134
CAACGCA701800.027.9758055
AACGCAG742850.026.4608356
GTACATG613450.025.2751521
TACATGG606500.025.0501272
ACGCAGA850350.023.0834927
ACATGGG631450.023.0045473
CGCAGAG850050.022.9903768
CATGGGT161200.022.4329934
GCAGAGT905000.021.338189
GAGTACT525350.020.43008412-13
CAGAGTA868050.020.23172810-11
CATGGGG319950.019.9035744
GTACTTT531750.019.55994614-15
Produced by FastQC (version 0.11.5)