FastQCFastQC Report
Thu 2 Jun 2016
DRR015187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21730745
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435690.2004947368348393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365530.16820868313534582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224350.10324082308268767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA407850.054.9388961
GTATCAA756300.042.3387761
GTGGTAT154000.034.0976031
TATCAAC986150.032.2941472
ATCAACG985700.032.188173
TCAACGC991700.032.0138784
CAACGCA1041350.030.4839255
TGGTATC169600.029.9131812
AACGCAG1093300.029.0952426
GTACATG1167700.027.0438081
TACATGG1166000.026.3716432
ACGCAGA1249250.025.4197337
CGCAGAG1272950.024.9379398
ACATGGG1192650.024.4759883
GCAGAGT1349700.023.1111569
CATGGGT293250.022.873154
CATGGGG613550.020.9295734
CATGGGA363850.020.0619144
CAGAGTA1296850.019.47004910-11
GAGTACA888450.019.3251841
Produced by FastQC (version 0.11.5)