FastQCFastQC Report
Thu 2 Jun 2016
DRR015187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21730745
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474580.21839103997584988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409130.18827242232146205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245660.11304720569865413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA428000.056.850781
GTATCAA803550.041.721091
GTGGTAT160600.032.4902341
ATCAACG1035050.032.176283
TATCAAC1040700.032.0817382
TCAACGC1043150.031.9061264
CAACGCA1086950.030.6433285
AACGCAG1147200.029.0897486
TGGTATC171850.028.9120452
GTACATG1188250.027.1293351
TACATGG1191500.026.4709932
ACGCAGA1318250.025.273737
CGCAGAG1342600.024.7719358
ACATGGG1223500.024.6250723
GCAGAGT1423700.023.0854919
CATGGGT301050.022.1806984
CATGGGG629950.021.6188964
GAGTACA905700.020.254741
CATGGGA374400.020.022084
AGTACAT864750.019.8939862
Produced by FastQC (version 0.11.5)