FastQCFastQC Report
Thu 2 Jun 2016
DRR015186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16264728
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305670.18793428331540496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264510.16262798861438077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA294800.056.7989271
GTATCAA551700.040.362671
GTGGTAT116500.031.7397961
ATCAACG698700.031.4583913
TCAACGC708100.031.0569214
TATCAAC713200.031.0114062
TGGTATC121150.029.4308622
CAACGCA755600.029.0785795
AACGCAG781800.028.2133546
GTACATG679800.025.5757751
TACATGG663250.025.1148822
ACGCAGA879650.025.0416977
CGCAGAG894500.024.5785248
ACATGGG694450.022.6939743
GCAGAGT958450.022.586189
CATGGGT183200.020.3298264
CAGAGTA911100.019.8416710-11
GAGTACT539050.019.56014412-13
CATGGGG377050.019.4778614
AGAGTAC863050.018.95660210-11
Produced by FastQC (version 0.11.5)