FastQCFastQC Report
Thu 2 Jun 2016
DRR015186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16264728
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321630.19774692819947556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296200.1821118680865736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171710.10557200833607547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA308200.057.941661
GTATCAA584100.040.8781741
ATCAACG746350.031.6027933
TCAACGC755400.031.2036134
TATCAAC760750.031.1407362
GTGGTAT118050.030.8560261
CAACGCA794300.029.6601875
TGGTATC119250.029.034182
AACGCAG822750.028.6622096
ACGCAGA937350.025.11437
GTACATG672350.024.7247811
CGCAGAG951150.024.659518
TACATGG663100.024.2545762
GCAGAGT1014750.022.7103589
ACATGGG690550.021.9958363
CAGAGTA966750.019.92406310-11
GAGTACT602100.019.68193212-13
CATGGGT178900.019.6795464
AGAGTAC926450.019.1334810-11
CATGGGG382450.019.0533944
Produced by FastQC (version 0.11.5)