FastQCFastQC Report
Thu 2 Jun 2016
DRR015185_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015185_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16448889
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT329210.20014117670804393Illumina RNA PCR Primer (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314780.19136854774811843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277720.16883815071036104No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC181400.11028100438880706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA308700.055.8291
GTATCAA574650.039.4624521
GTGGTAT117500.033.180371
ATCAACG715500.031.3365383
TGGTATC123200.031.1640782
TCAACGC727350.030.8615994
TATCAAC743450.030.3549392
CAACGCA795150.028.2213925
AACGCAG833200.027.0036816
GTACATG748500.025.7634751
TACATGG746900.025.23282
ACGCAGA914600.024.596527
CGCAGAG916600.024.486838
ACATGGG772500.023.2642693
GCAGAGT973700.022.6577329
CATGGGT176550.021.2292774
CATGGGG387650.020.0655544
CAGAGTA945150.019.63924610-11
CGTATCA98000.019.0080448-49
GAGTACT544650.019.00751312-13
Produced by FastQC (version 0.11.5)