FastQCFastQC Report
Thu 2 Jun 2016
DRR015185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16448889
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342880.2084517683838708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315050.19153269257273242No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT198650.12076803485025644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181350.11025060719906372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA325850.055.647981
GTATCAA599500.039.3009571
GTGGTAT125050.033.319871
TGGTATC127200.031.9081332
ATCAACG754000.030.9560853
TCAACGC765650.030.493984
TATCAAC777150.030.2053852
CAACGCA827500.028.2271295
AACGCAG865300.027.078986
AATCGTA15550.026.0358078
GTACATG765850.024.9968281
TACATGG758200.024.545012
ACGCAGA968550.024.1687327
CGCAGAG972650.023.9642568
ACATGGG780550.022.8163623
GCAGAGT1024400.022.4643929
CATGGGT183350.021.8310644
AAACTCG28600.020.199716
ATCGTAT25250.020.1769819
AACTCGT28150.019.5529887
Produced by FastQC (version 0.11.5)