FastQCFastQC Report
Thu 2 Jun 2016
DRR015184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17638117
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337260.19121088719391077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282820.16034591447601804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA324800.056.1563951
GTATCAA621450.041.4858171
ATCAACG783500.032.560773
TCAACGC787700.032.419544
TATCAAC794900.032.20322
GTGGTAT121700.030.760541
CAACGCA845600.030.1644885
AACGCAG887250.028.8407766
TGGTATC128850.028.2750592
GTACATG850600.026.465371
TACATGG839750.025.8736272
ACGCAGA997750.025.6120687
CGCAGAG999300.025.4887188
ACATGGG866650.023.8667853
GCAGAGT1067950.023.4532939
CATGGGT255050.023.1744334
CATGGGG436650.020.6069244
CAGAGTA1023950.020.00329810-11
GAGTACT576550.019.63685812-13
GAGTACA652900.019.0496831
Produced by FastQC (version 0.11.5)