FastQCFastQC Report
Thu 2 Jun 2016
DRR015184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17638117
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365210.20705724993206476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321200.18210560685134358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191750.10871341878500977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA334250.057.1066931
GTATCAA640200.041.2595371
ATCAACG818200.031.9358273
TCAACGC824600.031.7125764
GTGGTAT122100.031.6986261
TATCAAC834900.031.5106032
CAACGCA874750.029.9328255
TGGTATC128100.029.4809912
AACGCAG916150.028.6551536
GTACATG852200.026.042411
TACATGG847600.025.2945792
ACGCAGA1045400.025.0730177
CGCAGAG1047100.024.9675678
ACATGGG876500.023.370783
CATGGGT252400.023.0856634
GCAGAGT1118450.023.0652229
CATGGGG446000.020.3873394
GAGTACA660200.019.8151441
CAGAGTA1075550.019.69972810-11
GAGTACT628300.019.35393512-13
Produced by FastQC (version 0.11.5)