FastQCFastQC Report
Thu 2 Jun 2016
DRR015183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15300062
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT558880.3652795655337867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482300.3152274807775289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287150.18767897803289948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA389100.062.0726171
GTATCAA767650.043.4407841
GTGGTAT123750.039.6890071
TGGTATC133700.035.7137342
ATCAACG1003000.033.0415153
TATCAAC1007950.033.0073132
TCAACGC1018900.032.542174
CAACGCA1065650.031.1167835
AACGCAG1119550.029.762896
GTACATG883250.026.9945371
CGCAGAG1250900.026.5491168
TACATGG873700.026.4349982
ACGCAGA1264500.026.318587
GCAGAGT1322450.024.6606969
ACATGGG892000.024.569783
CATGGGT247750.023.294544
GAGTACT801350.021.34112412-13
CAGAGTA1281350.021.22013910-11
CATGGGG382550.021.153984
CATGGGA301700.020.6376064
Produced by FastQC (version 0.11.5)