FastQCFastQC Report
Thu 2 Jun 2016
DRR015183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15300062
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509970.3333123748125988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463600.3030053080830653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265870.17377053766187353No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT160650.10499957451152812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA411000.062.271191
GTATCAA807450.042.7938461
GTGGTAT128250.039.883541
TGGTATC134300.036.9399722
TATCAAC1069450.032.306862
ATCAACG1064400.032.2969133
TCAACGC1084650.031.711484
CAACGCA1124600.030.5891975
AACGCAG1178900.029.2536646
GTACATG892300.026.075531
CGCAGAG1342450.025.6991568
TACATGG880250.025.6319582
ACGCAGA1354000.025.5328047
ACATGGG895850.023.9960353
GCAGAGT1421850.023.9707439
CATGGGT242200.023.7189274
GAGTACT894400.020.95738412-13
CAGAGTA1382300.020.76213610-11
CATGGGG392350.020.3923364
AGAGTAC1329350.020.03158410-11
Produced by FastQC (version 0.11.5)