FastQCFastQC Report
Thu 2 Jun 2016
DRR015182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800312
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374180.3170933107531394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327820.2778062139373942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187960.1592839240182802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA279550.060.4648551
GTATCAA540900.041.7279551
GTGGTAT94900.036.6012461
TGGTATC95750.035.8476682
ATCAACG693000.032.276473
TATCAAC708150.031.7305952
TCAACGC707300.031.6343694
CAACGCA747500.029.92515
AACGCAG784550.028.7427226
GTACATG638500.026.4087351
TACATGG630600.025.9160522
CGCAGAG865900.025.914918
ACGCAGA876200.025.6849297
ACATGGG643600.023.9472163
GCAGAGT924500.023.8727329
CATGGGT170500.021.9164264
CAGAGTA899150.020.73688110-11
CATGGGG271000.020.5235674
GAGTACT557500.020.505712-13
CATGGGA215750.020.1255864
Produced by FastQC (version 0.11.5)