FastQCFastQC Report
Thu 2 Jun 2016
DRR015182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800312
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346110.2933058041177216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316560.2682640933561757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181050.15342814664561413No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT157350.1333439319231559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA304300.060.413361
GTATCAA569950.040.5694771
GTGGTAT103200.038.448511
TGGTATC103200.037.4913862
ATCAACG737350.031.1629563
TATCAAC752750.030.659252
TCAACGC752400.030.5829324
CAACGCA786800.029.2344075
AACGCAG831850.027.7560716
GTACATG651850.026.4841691
TACATGG645100.026.013412
CGCAGAG922400.024.971428
ACGCAGA936000.024.6943577
CATGGGT172600.024.1751124
ACATGGG662250.024.1140543
GCAGAGT981100.023.218669
CATGGGG272000.021.1702654
GAGTACT605850.021.01380712-13
CAGAGTA956400.020.69013210-11
GTACTTT607800.020.39618114-15
Produced by FastQC (version 0.11.5)