FastQCFastQC Report
Thu 2 Jun 2016
DRR015181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070259
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402030.2108151756093087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336270.17633216203303792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207960.10904938417459355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA367950.057.653081
GTATCAA708700.042.1565781
GTGGTAT135350.032.657761
ATCAACG911450.032.426093
TCAACGC922450.032.047524
TATCAAC934000.031.820832
TGGTATC138050.030.7979742
CAACGCA977350.030.2594835
AACGCAG1015600.029.2610116
GTACATG1023600.026.6881661
TACATGG1008100.026.3345322
ACGCAGA1140000.026.0122477
CGCAGAG1151850.025.7156358
CATGGGT371350.024.8566534
ACATGGG1027500.024.4888443
GCAGAGT1232850.023.6737949
CAGAGTA1206000.020.08095710-11
CATGGGG447750.020.0054174
GAGTACT672700.019.688912-13
AGAGTAC1153200.019.45414410-11
Produced by FastQC (version 0.11.5)