FastQCFastQC Report
Thu 2 Jun 2016
DRR015181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070259
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334070.17517853323334517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293560.15393603201718445No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA357050.056.6038321
GTATCAA690250.042.10331
GTGGTAT130450.033.7873731
ATCAACG889600.032.352113
TCAACGC902200.031.9057274
TATCAAC919450.031.4557762
CAACGCA944350.030.4865265
TGGTATC138550.030.475092
AACGCAG980900.029.4015056
GTACATG1031800.026.5004981
TACATGG1015550.026.1415862
ACGCAGA1133100.025.494157
CGCAGAG1146250.025.1822458
ACATGGG1032950.024.4327533
CATGGGT378050.024.3979174
GCAGAGT1229800.023.1951549
CATGGGG437800.020.4162314
ATGGGTA174250.019.8370535
CAGAGTA1201700.019.47866610-11
AGAGTAC1145250.019.31735810-11
Produced by FastQC (version 0.11.5)