FastQCFastQC Report
Thu 2 Jun 2016
DRR015180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15527451
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278950.17964957673992982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231510.14909723431102762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA275100.054.5887871
GTATCAA517300.040.6195451
ATCAACG646800.032.153163
TATCAAC650450.032.0870742
TCAACGC649950.032.030074
GTGGTAT109250.030.583441
CAACGCA701550.029.6333795
AACGCAG739100.028.218676
TGGTATC118600.027.1356012
GTACATG669050.025.8885541
ACGCAGA811950.025.643797
TACATGG665600.025.3862972
CGCAGAG824600.025.2327028
ACATGGG686500.023.5507583
GCAGAGT876400.023.1176479
CATGGGG385300.020.3411664
CAGAGTA824400.019.86942910-11
GAGTACT459650.019.70620212-13
AGAGTAC785400.019.17067110-11
CATGGGT139200.019.1275064
Produced by FastQC (version 0.11.5)