FastQCFastQC Report
Thu 2 Jun 2016
DRR015180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15527451
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263220.16951913098936844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225480.1452137894365276No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT157280.10129157709143632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277450.055.0497551
GTATCAA527400.040.8089681
TATCAAC672400.031.7305092
ATCAACG671550.031.6147823
TCAACGC676700.031.3815824
GTGGTAT109250.030.1773431
CAACGCA716800.029.6576045
AACGCAG750450.028.4130236
TGGTATC117300.027.2312242
ACGCAGA855700.024.9729617
GTACATG679700.024.7528171
CGCAGAG868150.024.5429578
TACATGG672600.024.2168772
GCAGAGT923100.022.7236219
ACATGGG694450.022.4431463
CATGGGT144650.019.6392634
GAGTACT500700.019.31429912-13
CAGAGTA881800.019.31402210-11
CATGGGG384350.019.15594
AGAGTAC842400.018.94268210-11
Produced by FastQC (version 0.11.5)