FastQCFastQC Report
Thu 2 Jun 2016
DRR015179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21220152
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370490.17459347133799985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303390.14297258568176138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA348250.054.6236531
GTATCAA666050.042.5023771
ATCAACG849450.032.9575653
TCAACGC855450.032.7517744
TATCAAC867300.032.4451642
CAACGCA911150.030.7433075
GTGGTAT132650.029.8203011
AACGCAG952300.029.5131956
GTACATG959900.027.0965351
TACATGG954500.026.3574752
TGGTATC146700.026.094932
ACGCAGA1077450.026.043247
CGCAGAG1104350.025.3253968
ACATGGG977050.024.3817123
GCAGAGT1186100.023.0224699
CATGGGT278000.022.10144
CATGGGG524300.020.7727364
CAGAGTA1130200.019.42381310-11
AGAGTAC1070700.019.20317610-11
GAGTACA753800.019.1487141
Produced by FastQC (version 0.11.5)