FastQCFastQC Report
Thu 2 Jun 2016
DRR015179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21220152
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342390.1613513418754022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291440.1373411462839663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA370100.055.527321
GTATCAA701600.042.560051
ATCAACG907450.032.560763
TCAACGC911500.032.426874
TATCAAC922250.032.1671682
GTGGTAT135000.031.2381361
CAACGCA954950.030.970655
AACGCAG1002550.029.542476
TGGTATC152500.026.529832
GTACATG979850.026.3554271
ACGCAGA1151000.025.7655967
TACATGG971700.025.7650812
CGCAGAG1178000.025.1445168
ACATGGG992150.023.8814563
GCAGAGT1260300.023.1717019
CATGGGT281350.022.1896294
CATGGGG528250.020.5663594
CAGAGTA1202850.019.66337810-11
AGAGTAC1145200.019.34572410-11
GAGTACA771400.019.1046751
Produced by FastQC (version 0.11.5)