FastQCFastQC Report
Thu 2 Jun 2016
DRR015178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485783
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346750.3018949600562713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308820.268871525781046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177240.15431250964779675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273400.058.8428761
GTATCAA517900.041.408841
TGGTATC94900.036.3594672
GTGGTAT98850.035.4140471
TATCAAC660950.032.2805442
ATCAACG659400.032.2294923
TCAACGC672400.031.6182614
CAACGCA712400.029.841135
AACGCAG753050.028.3913696
CGCAGAG825150.025.8388868
GTACATG545500.025.5943951
ACGCAGA835650.025.5590027
TACATGG539700.025.0337542
GCAGAGT874550.023.8056079
ACATGGG551950.023.30513
CATGGGT122000.021.1533454
GAGTACT523650.020.99381812-13
CAGAGTA831150.020.983410-11
CATGGGG251150.020.5330074
GTACTTT538300.019.83522414-15
Produced by FastQC (version 0.11.5)