FastQCFastQC Report
Thu 2 Jun 2016
DRR015178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485783
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328490.28599704521668223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303200.2639785202280071No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174440.15187471328685212No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT144860.12612113601658678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA284800.060.155091
GTATCAA537050.040.9522171
TGGTATC95000.040.488832
GTGGTAT97550.040.396421
TATCAAC698550.031.3127272
ATCAACG699200.031.1467343
TCAACGC711700.030.5878854
CAACGCA744900.029.2309275
AACGCAG791700.027.5775056
GTACATG558800.024.9701711
CGCAGAG879500.024.8235958
ACGCAGA891100.024.550227
TACATGG552850.024.2651082
GCAGAGT933600.023.0841569
ACATGGG559500.022.5104663
GAGTACT587500.020.5783912-13
CATGGGT123450.020.4784054
CAGAGTA897800.020.4001310-11
CATGGGG248900.019.749584
GTACTTT594550.019.66539414-15
Produced by FastQC (version 0.11.5)