FastQCFastQC Report
Thu 2 Jun 2016
DRR015177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17596192
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417230.2371138028046068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360700.20498753366637507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220550.12533961893573337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA356350.057.5872881
GTATCAA670000.041.676231
GTGGTAT128800.034.8835561
TGGTATC136550.032.268932
ATCAACG868100.031.8368133
TCAACGC878500.031.4638464
TATCAAC881950.031.463132
CAACGCA932500.029.6407995
AACGCAG978750.028.3588716
GTACATG828250.026.1946181
TACATGG814250.025.8556182
ACGCAGA1100450.025.194027
CGCAGAG1100300.025.1258648
ACATGGG847200.023.6607723
GCAGAGT1172450.023.1321989
CATGGGT218650.021.669634
CATGGGG423000.021.0356854
CAGAGTA1109650.020.09702310-11
GAGTACT659800.019.9564212-13
AGAGTAC1054750.019.4956210-11
Produced by FastQC (version 0.11.5)