FastQCFastQC Report
Thu 2 Jun 2016
DRR015177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17596192
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368420.20937484655770977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332400.18890450843000575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195490.11109790118225579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA367700.058.4549261
GTATCAA688900.040.540621
GTGGTAT126150.034.4002081
TGGTATC132000.031.6440412
ATCAACG897450.030.8074473
TATCAAC910450.030.5376052
TCAACGC907950.030.4670934
CAACGCA951200.029.0818715
AACGCAG997800.027.7759046
GTACATG846350.025.6852861
TACATGG835150.025.132332
ACGCAGA1139450.024.3489257
CGCAGAG1142050.024.2573078
ACATGGG860700.023.1749863
GCAGAGT1211900.022.5152159
CATGGGT217700.020.9559444
CATGGGG435950.020.648924
GAGTACT683500.019.97075512-13
CAGAGTA1151400.019.96504610-11
AGAGTAC1101050.019.25271810-11
Produced by FastQC (version 0.11.5)