FastQCFastQC Report
Thu 2 Jun 2016
DRR015176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17626553
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288560.16370756097349265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255420.14490638073138862No Hit
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT240370.1363681259744886Illumina Single End PCR Primer 1 (100% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA298100.054.7752271
GTATCAA552500.040.8909071
ATCAACG707650.031.5743923
TATCAAC714000.031.481942
TCAACGC711300.031.4550194
GTGGTAT121500.030.0478131
CAACGCA766900.029.1917635
AACGCAG802300.028.004256
TGGTATC125100.027.6544422
GTACATG717250.025.5706351
ACGCAGA886750.025.2616737
TACATGG708300.025.1028842
CGCAGAG896950.024.9525498
ACATGGG740300.022.9207423
GCAGAGT952850.022.8762329
CAGAGTA913400.019.71148710-11
CATGGGG430050.019.7056884
CATGGGT170100.019.6123924
GAGTACT529600.019.19045312-13
AGAGTAC869650.019.10514510-11
Produced by FastQC (version 0.11.5)