FastQCFastQC Report
Thu 2 Jun 2016
DRR015176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17626553
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282600.16032629862458078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253180.1436355707210593No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA310900.055.191121
GTATCAA585400.041.651071
ATCAACG759000.031.8142013
TATCAAC764850.031.6909522
TCAACGC764500.031.5745474
GTGGTAT119600.031.2037981
CAACGCA812400.029.718965
AACGCAG847900.028.496466
TGGTATC127000.028.4323272
ACGCAGA961600.025.1862747
GTACATG733350.025.0984331
CGCAGAG970600.024.8929738
TACATGG723650.024.5923862
GCAGAGT1027450.023.308379
ACATGGG760250.022.4836063
CATGGGT176900.020.078844
CAGAGTA990150.019.7125310-11
GAGTACT591700.019.4025112-13
AGAGTAC941250.019.38644610-11
CATGGGG437650.019.3167974