FastQCFastQC Report
Thu 2 Jun 2016
DRR015175_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015175_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16033115
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246530.1537630086230904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209340.13056726656049059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA293300.055.3256451
GTATCAA544950.041.774781
ATCAACG700650.032.1323853
TATCAAC712400.031.7701572
TCAACGC712500.031.6015014
GTGGTAT116150.030.575691
CAACGCA761300.029.5675325
TGGTATC120150.028.3829482
AACGCAG802100.028.129296
GTACATG801850.026.3920961
TACATGG789450.025.8261072
CGCAGAG890400.025.2664688
ACGCAGA895600.025.135717
CATGGGT264800.024.3607334
ACATGGG809550.024.086973
GCAGAGT959400.023.0840979
CATGGGG387650.020.2693354
CAGAGTA914800.019.77696410-11
GAGTACT467200.019.19103412-13
AGAGTAC864150.019.15417510-11
Produced by FastQC (version 0.11.5)