FastQCFastQC Report
Thu 2 Jun 2016
DRR015175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16033115
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289570.18060744902035566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262140.16349910794003536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA323250.055.8248441
GTATCAA585700.041.0488171
GTGGTAT131250.031.5013031
ATCAACG767700.031.0423
TGGTATC128350.030.9234312
TATCAAC779100.030.7181072
TCAACGC778800.030.6094424
CAACGCA817500.029.1489125
AACGCAG866500.027.6025686
GTACATG809700.025.0083451
TACATGG801150.024.463992
CGCAGAG984500.024.2235818
ACGCAGA992200.024.067997
ACATGGG824500.022.7276423
GCAGAGT1052100.022.406669
CATGGGT273150.022.0556414
GAGTACT533900.019.98038112-13
CATGGGG385250.019.7245354
CAGAGTA1001000.019.6211910-11
AGAGTAC950100.019.0107210-11
Produced by FastQC (version 0.11.5)