FastQCFastQC Report
Thu 2 Jun 2016
DRR015174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26140372
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441510.16889966217772265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355130.13585499089301406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA438650.055.2625271
GTATCAA841700.042.7671471
ATCAACG1082300.032.815523
TATCAAC1093950.032.6016652
TCAACGC1090550.032.5764924
CAACGCA1157150.030.7068275
GTGGTAT169000.030.5248831
AACGCAG1224150.029.1012766
TGGTATC183950.027.227142
GTACATG1253300.026.5021481
TACATGG1238100.025.9300962
ACGCAGA1380850.025.7552667
CGCAGAG1399750.025.3444428
ACATGGG1282100.023.973033
GCAGAGT1485800.023.4019839
CATGGGT346250.022.9160734
CATGGGG693250.020.1201634
CAGAGTA1404950.019.87167510-11
AGAGTAC1334850.019.4021910-11
GAGTACA950650.019.2825261
Produced by FastQC (version 0.11.5)