FastQCFastQC Report
Thu 2 Jun 2016
DRR015174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26140372
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447410.17115670733377475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378860.14493290302066092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA454950.054.858581
GTATCAA846800.041.5238271
GTGGTAT178900.031.9689041
ATCAACG1094950.031.7426833
TCAACGC1103300.031.5339974
TATCAAC1108300.031.4909762
CAACGCA1162350.029.95145
AACGCAG1229450.028.3878776
TGGTATC190400.028.3038882
GTACATG1260750.026.4236681
TACATGG1243650.025.8092562
ACGCAGA1408550.024.7287147
CGCAGAG1426750.024.4479688
ACATGGG1282050.023.8412323
GCAGAGT1521400.022.52249
CATGGGT342750.022.409564
CATGGGG701700.020.3488484
CAGAGTA1441400.019.37947310-11
GAGTACA962900.019.2658541
AGAGTAC1375300.018.95617310-11
Produced by FastQC (version 0.11.5)