FastQCFastQC Report
Thu 2 Jun 2016
DRR015173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12820950
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328950.25657225088624475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294740.22988936077279767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174980.13647974604066002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA283000.055.2183461
GTATCAA507200.038.4675181
GTGGTAT117950.033.8165051
TGGTATC116300.033.0825962
TATCAAC645100.030.1174472
ATCAACG644950.030.002093
TCAACGC653100.029.6530344
CAACGCA724200.026.7582345
AACGCAG742750.026.1501276
ACGCAGA799050.024.2728927
GTACATG541850.024.0330851
CGCAGAG812650.023.8208648
TACATGG536100.023.6028982
GCAGAGT852450.022.3564579
ACATGGG564050.021.5517373
CATGGGG321150.020.342084
GAGTACT493900.020.15605412-13
CAGAGTA811450.020.13955910-11
GTACTTT513950.018.79049114-15
AGTACTT493750.018.62824812-13
Produced by FastQC (version 0.11.5)