FastQCFastQC Report
Thu 2 Jun 2016
DRR015173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12820950
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365890.2853844683896279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344440.2686540388972736No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT267100.20833089591644924No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG253850.19799624832793203RNA PCR Primer, Index 39 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203070.15838919892831654No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT166290.12970177716939854RNA PCR Primer, Index 39 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301850.056.598181
GTATCAA534850.038.4716761
GTGGTAT118150.032.8349721
TGGTATC115500.032.4312632
TATCAAC687450.029.8594262
ATCAACG683600.029.841773
TCAACGC692450.029.4390494
CAACGCA748700.027.2393365
AACGCAG767950.026.613196
ACGCAGA854350.023.9208767
CGCAGAG865850.023.597368
GTACATG551850.023.3257451
TACATGG540500.023.18232
GCAGAGT907550.022.1666139
ACATGGG579250.020.7943733
GAGTACT542150.020.60391612-13
CAGAGTA867250.020.31869710-11
GTACTTT563400.019.33955814-15
CATGGGG324650.019.1316054
AGTACTT538800.018.98781212-13
Produced by FastQC (version 0.11.5)