FastQCFastQC Report
Thu 2 Jun 2016
DRR015172_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015172_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14704647
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378260.25723840905531425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333030.2264794251776326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196520.1336448267000221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA330300.057.3106161
GTATCAA602250.040.7144621
GTGGTAT122300.033.9862671
TGGTATC121800.033.0792122
ATCAACG778550.031.3758343
TATCAAC783100.031.294382
TCAACGC790750.030.8718574
CAACGCA844650.028.894075
AACGCAG883100.027.7179496
GTACATG684350.024.9732571
ACGCAGA984700.024.8114227
CGCAGAG982700.024.786838
TACATGG678300.024.4241472
GCAGAGT1033950.023.175419
ACATGGG701450.022.279643
GAGTACT585500.020.76321412-13
CATGGGT197400.020.753414
CAGAGTA988250.020.46115510-11
GTACTTT595400.019.70413414-15
CATGGGG330600.019.4137954
Produced by FastQC (version 0.11.5)