FastQCFastQC Report
Thu 2 Jun 2016
DRR015172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14704647
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436580.29689934073221885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401670.27315854641053267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231280.15728361245258046No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT211210.14363486590327534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA353600.057.193671
GTATCAA647400.040.4789961
GTGGTAT134150.034.990461
TGGTATC130700.034.6470762
ATCAACG836100.031.0907023
TATCAAC840850.030.9705142
TCAACGC850250.030.5780124
CAACGCA897400.029.0118895
AACGCAG940200.027.7275586
CGCAGAG1056750.024.578218
ACGCAGA1059450.024.541347
GTACATG696050.023.9922281
TACATGG687100.023.3882032
GCAGAGT1112950.023.0500149
ACATGGG705400.021.5251063
CATGGGT196750.020.5821594
GAGTACT661950.020.56185712-13
CAGAGTA1064800.020.25746510-11
GTACTTT673050.019.6125614-15
AGAGTAC1019150.019.1291610-11
Produced by FastQC (version 0.11.5)