FastQCFastQC Report
Thu 2 Jun 2016
DRR015171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22418155
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351420.15675687852100229No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291310.12994378886219674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA377850.052.787741
GTATCAA707800.041.9066621
ATCAACG900350.032.536863
TATCAAC912100.032.3002052
TCAACGC909250.032.2613534
GTGGTAT149850.030.655871
CAACGCA966200.030.3340765
AACGCAG1004950.029.26826
TGGTATC168500.026.2899192
ACGCAGA1137950.025.7827077
GTACATG965750.025.6032051
CGCAGAG1159250.025.2257778
TACATGG951700.024.957272
GCAGAGT1242100.023.110999
ACATGGG991250.022.8847523
CATGGGG552950.019.576564
CATGGGT234350.019.4437274
CAGAGTA1172650.019.4177410-11
AGAGTAC1100850.019.28129410-11
GAGTACT640300.018.98263712-13
Produced by FastQC (version 0.11.5)