FastQCFastQC Report
Thu 2 Jun 2016
DRR015171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22418155
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384400.17146816943678014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336210.14997219887185187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA397550.054.297691
GTATCAA730350.041.0638581
ATCAACG930500.031.8707393
TATCAAC936400.031.8130262
TCAACGC937500.031.635724
GTGGTAT150800.030.3738441
CAACGCA986650.030.0273675
AACGCAG1028650.028.8555016
TGGTATC169200.025.6628422
GTACATG970650.025.433141
TACATGG958400.025.0419252
ACGCAGA1189450.024.9465857
CGCAGAG1213550.024.4431258
ACATGGG998050.022.8426383
GCAGAGT1300950.022.4573279
CATGGGG554900.019.8360774
CAGAGTA1229600.019.4000410-11
GAGTACT690300.019.16165412-13
AGAGTAC1161150.018.8725210-11
CATGGGT234550.018.837294
Produced by FastQC (version 0.11.5)