FastQCFastQC Report
Thu 2 Jun 2016
DRR015170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9743165
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346150.3552746976983352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330900.33962269960531305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177640.18232268467176735No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105680.10846578088331667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA302500.058.8652341
GTGGTAT102300.039.7885671
GTATCAA552000.039.4097331
TGGTATC106100.037.8485872
ATCAACG708950.030.5279463
TATCAAC712100.030.510962
TCAACGC722150.030.0180574
CAACGCA776050.027.9433255
AACGCAG802200.027.0755446
CGCAGAG889050.024.3167428
ACGCAGA890850.024.2990027
GTACATG662150.023.7430571
TACATGG652600.023.5463282
GCAGAGT942300.022.6816439
ACATGGG656400.021.8957613
TCTGTCG27500.021.5063068
CATGGGT101150.021.3320714
GAGTACT542900.020.7410812-13
GTACTTT539950.020.31987214-15
CAGAGTA940250.020.2154910-11
Produced by FastQC (version 0.11.5)