FastQCFastQC Report
Thu 2 Jun 2016
DRR015170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9743165
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393710.4040884045379505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393230.40359575148321924No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT212420.2180195039291647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201040.20633952108991277No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT135850.13943107809423325RNA PCR Primer, Index 26 (100% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125520.12882877381220578No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG114400.11741564471093326RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA317850.061.483761
GTGGTAT98750.041.590331
GTATCAA593850.040.8670581
TGGTATC100000.040.3280452
CGGTATC5750.032.4555852
TATCAAC773750.031.2898032
ATCAACG773500.031.2219243
TCAACGC788100.030.6873514
CAACGCA831100.029.1046545
AACGCAG859450.028.1573036
CGCAGAG966300.025.004698
ACGCAGA967450.024.97987
GCTCTAG18800.024.4616891
GTACATG671500.023.9427831
GCGGTAT8200.023.8768791
TACATGG661400.023.721962
GCAGAGT1021600.023.4015469
ACATGGG664950.022.3031523
CATGGGT105000.021.9279824
GAGTACT618700.021.0118912-13
Produced by FastQC (version 0.11.5)