FastQCFastQC Report
Thu 2 Jun 2016
DRR015169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14768587
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278100.18830508294395396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251700.1704293037648084No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT202650.13721691858537313Illumina RNA PCR Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA294150.055.102311
GTATCAA521400.039.5388531
GTGGTAT113850.031.7523691
ATCAACG661550.030.933093
TATCAAC665900.030.7948462
TCAACGC667800.030.6686694
TGGTATC121750.028.4953942
CAACGCA728150.028.118185
AACGCAG767300.026.7357126
ACGCAGA835600.024.5059267
CGCAGAG839750.024.2867248
GTACATG608900.023.6879711
TACATGG600150.023.2216032
GCAGAGT896550.022.3877149
ACATGGG631050.021.058463
GAGTACT488750.020.53600312-13
CAGAGTA845300.020.3095410-11
GTACTTT519350.018.86651214-15
AGAGTAC801500.018.68948210-11
AGTACTT498800.018.44880312-13
Produced by FastQC (version 0.11.5)