FastQCFastQC Report
Thu 2 Jun 2016
DRR015169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14768587
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296710.2009061530395562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277690.18802746667639902No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT211860.14345312791264325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156770.10615098113313075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303650.054.2671281
GTATCAA543400.039.8849331
GTGGTAT121200.033.4355851
TGGTATC126200.031.4512122
ATCAACG693800.030.9656283
TATCAAC700950.030.7931962
TCAACGC701850.030.6024254
CAACGCA755650.028.4473275
AACGCAG790350.027.2574426
GTACATG622900.024.9335961
CGCAGAG877550.024.526878
ACGCAGA879100.024.4995657
TACATGG617050.024.2765942
ACTCGTA24350.023.1677468
GCAGAGT938400.022.6347069
ACATGGG648200.022.2340853
AATCGTA17000.022.2121328
GAGTACT521050.020.75765812-13
ATCGTAT25100.020.118169
CAGAGTA888400.020.07810210-11
Produced by FastQC (version 0.11.5)