FastQCFastQC Report
Thu 2 Jun 2016
DRR015168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18988499
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399210.2102377865675428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334940.17639098277330925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207010.10901862227235551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA368400.056.6545941
GTATCAA703300.041.1031271
GTGGTAT145500.032.356961
ATCAACG897400.032.042483
TCAACGC910650.031.608344
TATCAAC915450.031.5078452
TGGTATC147000.031.0973322
CAACGCA968600.029.7380125
AACGCAG995300.028.9765156
GTACATG852250.026.1140581
ACGCAGA1118900.025.7297237
TACATGG842000.025.5989932
CGCAGAG1134350.025.3219328
ACATGGG878200.023.4685973
GCAGAGT1207600.023.367879
CATGGGG410900.020.6730964
CATGGGT203450.020.2721824
CAGAGTA1164450.020.0437410-11
GAGTACT690500.019.61724312-13
AGAGTAC1102750.019.34609610-11
Produced by FastQC (version 0.11.5)