FastQCFastQC Report
Thu 2 Jun 2016
DRR015168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18988499
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452580.23834427355211174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406930.21430340544558052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243370.12816705522642943No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT192680.10147194888864043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA394000.058.440181
GTATCAA742900.040.4754371
GTGGTAT147350.033.4033781
TGGTATC147500.032.1540762
ATCAACG944800.031.5428963
TCAACGC956600.031.157254
TATCAAC961650.031.1104952
CAACGCA1008300.029.5504195
AACGCAG1039950.028.651986
GTACATG857250.025.8133031
TACATGG847450.025.1948412
ACGCAGA1187150.025.0715397
CGCAGAG1202200.024.7311238
ACATGGG880500.023.0603923
GCAGAGT1285150.022.8044179
CATGGGT201350.020.9948254
CAGAGTA1240500.019.9414610-11
GAGTACT771900.019.87518312-13
CATGGGG411650.019.3997964
GTACTTT761850.019.26052714-15
Produced by FastQC (version 0.11.5)