FastQCFastQC Report
Thu 2 Jun 2016
DRR015167_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015167_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20282746
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400730.19757186724125028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341850.16854226740304296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206960.10203746573565532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA371950.054.654561
GTATCAA684850.041.3574941
ATCAACG879350.031.8918323
TCAACGC888850.031.5531374
TATCAAC898150.031.352932
GTGGTAT146000.030.2488161
CAACGCA960850.029.1995725
AACGCAG999950.028.1318026
TGGTATC152800.027.9192372
GTACATG875500.026.6466391
TACATGG867450.026.0697612
ACGCAGA1117650.025.115457
CGCAGAG1122250.024.9270658
ACATGGG907350.023.898263
GCAGAGT1185900.023.1633459
CATGGGG500050.021.2656784
CATGGGT237800.021.172064
GAGTACA675350.020.1370491
CAGAGTA1140550.019.93640510-11
GAGTACT687350.019.9035212-13
Produced by FastQC (version 0.11.5)