FastQCFastQC Report
Thu 2 Jun 2016
DRR015167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20282746
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441660.2177515805798682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393630.19407135503249906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238110.1173953467641906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA412550.055.526741
GTATCAA745200.040.7730031
ATCAACG960350.031.3220333
GTGGTAT153800.031.115071
TCAACGC971500.030.9804424
TATCAAC982550.030.735962
CAACGCA1029800.029.2308945
TGGTATC162100.028.8087772
AACGCAG1071400.028.1727356
GTACATG895300.025.8966881
TACATGG887100.025.2744852
ACGCAGA1214050.024.827937
CGCAGAG1219850.024.6648838
ACATGGG935800.023.054993
GCAGAGT1292900.022.9148569
CATGGGT244400.021.356424
GAGTACT767150.020.41419212-13
CATGGGG511700.020.4095544
CAGAGTA1240500.020.15102210-11
AGAGTAC1189850.019.42378810-11
Produced by FastQC (version 0.11.5)