FastQCFastQC Report
Thu 2 Jun 2016
DRR015166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20381972
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420920.2065158366423033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355960.17464453390476642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216450.10619678998675888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA395500.057.4579351
GTATCAA774200.042.526561
GTGGTAT142450.033.4517751
ATCAACG986600.033.0280043
TATCAAC1000800.032.705142
TCAACGC999550.032.6075254
CAACGCA1037800.031.4392495
AACGCAG1084000.030.189596
TGGTATC152750.030.03262
GTACATG1012900.027.1666771
TACATGG1003650.026.4906652
ACGCAGA1243200.026.2860457
CGCAGAG1258850.025.8976828
ACATGGG1037100.024.2888763
GCAGAGT1343200.023.8818049
CATGGGT312250.022.0362324
CATGGGG509000.021.0195224
CAGAGTA1282500.020.13675310-11
GAGTACT737750.019.65936312-13
AGAGTAC1217550.019.58766710-11
Produced by FastQC (version 0.11.5)