FastQCFastQC Report
Thu 2 Jun 2016
DRR015166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20381972
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403020.1977335657217074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352870.1731284882542278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210770.10341001351586589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA411700.058.382121
GTATCAA795000.041.9402431
GTGGTAT149100.033.2857131
ATCAACG1015950.032.4492343
TATCAAC1032150.032.074692
TCAACGC1027450.032.07374
CAACGCA1064150.030.9464825
TGGTATC154200.030.8097692
AACGCAG1111100.029.6913176
GTACATG1017900.026.2299751
ACGCAGA1281550.025.7270057
TACATGG1015450.025.4274652
CGCAGAG1295350.025.4035578
GCAGAGT1385050.023.4331729
ACATGGG1048600.023.3643843
CATGGGT313150.021.4594884
CATGGGG521300.020.1524334
CAGAGTA1321200.020.08870710-11
GAGTACT753950.019.8082412-13
AGAGTAC1247700.019.56172810-11
Produced by FastQC (version 0.11.5)