FastQCFastQC Report
Thu 2 Jun 2016
DRR015165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13801128
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297380.21547514087254319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255920.18543411813874922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152860.1107590625925649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA280250.056.642661
GTATCAA542250.042.157651
ATCAACG698300.032.39693
TATCAAC704550.032.232592
TCAACGC702750.032.177994
GTGGTAT111050.031.966041
TGGTATC112700.030.3668962
CAACGCA748250.030.2680785
AACGCAG785100.028.8976736
GTACATG735500.026.2420861
TACATGG726950.025.842922
ACGCAGA887400.025.5608357
CGCAGAG886800.025.547258
ACATGGG748400.023.9405083
GCAGAGT941150.023.6897359
CATGGGG357450.021.4905644
CATGGGT153450.020.3148864
GAGTACT478500.020.26045612-13
CAGAGTA902250.020.01811610-11
CATGGGA278000.019.9712734
Produced by FastQC (version 0.11.5)